Renal microsomal N-nitrosodimethylamine demethylase determined by a sensitive radiometric method.
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چکیده
منابع مشابه
Regulation of renal N-nitrosodimethylamine demethylase activity by androgens, progestin, glucocorticoid, and estrogen in BALB/c mice.
Studies from this laboratory have shown that renal N-nitrosodimethylamine demethylase (NDMA demethylase), an enzyme responsible for the metabolism of NDMA, shows age- and sex-related differences and is modulated by testosterone. These results have been positively correlated with NDMA-induced mutagenicity and carcinogenicity studies; however, relatively large doses of testosterone (100 mg/kg or ...
متن کاملInduction of rat hepatic N-nitrosodimethylamine demethylase by acetone is due to protein stabilization.
The N-nitrosodimethylamine demethylase (P450I-IE1) is induced severalfold in liver by giving rats ethanol, acetone, pyrazole, and other related small molecular weight compounds. This induction is not the result of an increase in IIE1 mRNA, but could be due to either an increase in translation rate or a decrease in protein degradation. To determine the mechanism of induction, we measured IIE1 sy...
متن کاملRegulation of A^-Nitrosodimethylamine Demethylase in Rat Liver and Kidney1
In previous work, the low k„, form of /V-nitrosodimethylamine (NDMA) demethylase has been demonstrated to be due to a specific form of cytochrome P-450 (designated as P-450ac) and to be the enzyme required for the metabolic activation of NDMA. The present work deals with the regulation of P-450ac in rat liver during development as well as the mechanism of induction of P-450ac in rat liver and...
متن کاملEnhancement of rat liver microsomal metabolism of azoxymethane to methylazoxymethanol by chronic ethanol administration: similarity to the microsomal metabolism of N-nitrosodimethylamine.
We compared the metabolism of azoxymethane (AOM) and of N-nitrosodimethylamine (NDMA) by liver microsomes obtained from male F344 rats pair-fed for 3 weeks either a control liquid diet or an isocaloric liquid diet containing ethanol at a concentration of 6.6% by volume. High-performance liquid chromatographic analysis of the products of the microsomal metabolism of AOM showed that methylazoxyme...
متن کاملInhibition of N-nitrosodimethylamine metabolism in rats by ether anesthesia.
Short-term exposure to diethyl ether strongly inhibits the metabolism of N-nitrosodimethylamine (NDMA). Twenty-six 6-week-old male Fischer 344 rats were exposed to ether vapor until their righting reflex was lost (approximately 2 min). The animals were removed from the ether and NDMA was immediately administered by i.v. bolus injection at a dose of 300 microgram/kg via a cannula surgically inse...
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ژورنال
عنوان ژورنال: Journal of Pharmacobio-Dynamics
سال: 1984
ISSN: 0386-846X,1881-1353
DOI: 10.1248/bpb1978.7.872